The third USS Seneca (SP-427), later USS SP-427, was a United States Navy minesweeper and patrol vessel in commission from 1917 to 1919.

Seneca was built as a civilian steam yacht in 1888 at Boston, Massachusetts.

On 7 May 1917, the U.S. Navy acquired her from her owner, the Johnson Lighterage Company, for use as a minesweeper and patrol vessel on the section patrol during World War I.

Based at Tompkinsville, Staten Island, New York, Seneca carried out minesweeping and patrol duties for the rest of World War I.

She was stricken from the Navy List on 6 January 1919 and returned to Johnson Lighterage the same day.