Up the MacGregors!

[3] The film has the same cast as its predecessor except for Manuel Zarzo and Robert Woods, who refused the role due to his conflicts with the leading actress Agata Flori,[3] the wife of producer Dario Sabatello.

The elder MacGregors wakes up in the middle of the night to take out a chest of gold ingots and coins from the hiding place and bury it outside their house while being secretly watched by a bandit spy.

They left a note, purporting to be from Frank James, in the hole where the chest of gold was buried.

Upon finding out that Gregor has gone with the traveling medicine man and his daughter, Dolly, to Maldonado's hide out, Rosita out of jealousy ride out to confront Dolly; ending up being kept as a prisoner of the outlaws together with Gregor who came back to rescue her after he and his brothers managed to regain the stolen gold.

[4] In a contemporary review, Stuart Byron of Variety found the film to be "an acceptably entertaining affair" while noting the film was bloodless and non-violent but had good action scenes directed by Franco Giraldi.