Use of DNA in forensic entomology

With the magnitude of information that can be gathered, investigators can more accurately determine time of death, location, how long a body has been in a specific area, if it has been moved, and other important factors.

When it is time for analysis, the DNA must then be extracted by dissecting the posterior end of the abdomen and collecting 25 mg of tissue.

Once extracted and isolated, the DNA sample goes through a polymerase chain reaction (PCR), is amplified and identified.

The following among the flies (Diptera) have been utilized:[2] Listed here are fleas commonly encountered by humans that could potentially be used for DNA identification.

Testing a sample of a residence's bed bug population and screening for bites could reveal possible recent visitors to the structure, as they have been observed to feed approximately once a week in temperate conditions.

[9] Recent studies have revealed that human DNA can be recovered from bed bugs for up to 60 days after feeding, thus demonstrating the potential use of this insect in forensic entomology [10][11] Lice can be indicators of contact with another person.

DNA identification of multiple individuals using blood meals from body and head lice has been demonstrated in laboratory settings.

[12] Due to the low probability of a tick detaching and falling to the ground at the scene of the crime, these may not be highly useful regardless of the large amount of blood and lymph they ingest.

This DNA can be obtained from practically any part of the insect, including the body, leg, setae, antennae, etc.