Ussing chamber

[1] The technique is used to measure the short-circuit current as an indicator of net ion transport taking place across an epithelium.

Ussing chambers are used to measure ion transport in native tissue, such as gut mucosa, and in a monolayer of cells grown on permeable supports.

The Ussing chamber provides a system to measure the transport of ions, nutrients, and drugs across various epithelial tissues,[2] (although can generate false-negative results for lipophilic substances[3]).

It consists of two halves separated by the epithelia (sheet of mucosa or monolayer of epithelial cells grown on permeable supports).

The two half chambers are filled with equal amounts of symmetrical Ringer solution to remove chemical, mechanical or electrical driving forces.