In the fall of 1961, the squadron was called upon to assist in the evacuation and resupply of victims of Hurricane Hattie, which hit Belize.

On 28 April 1965, HMM-264 conducted the Marine Corps' first night all-helicopter assault into an unsecured landing zone during combat conditions in Santa Domingo, Dominican Republic from the deck of the USS Boxer during Operation Power Pack.

Over the next several years, the squadron maintained its demonstrated high standard of professional excellence by logging 30,000, 40,000, and 50,000 mishap free flight hour milestones.

In 1968, the squadron retired the last of its aging fleet of UH-34 helicopters, receiving in its place the Boeing-Vertol CH-46 Sea Knight aircraft.

Throughout the early 1980s, the Black Knights were frequent visitors to the Mediterranean as the Aviation Combat Element (ACE) for deploying east coast Marine Amphibious Units supporting Landing Force Sixth Fleet (LF6F) commitments.

During the first two weeks of Operation Provide Comfort, the Black Knights flew more than 1000 hours delivering more than 450 tons of relief supplies to the isolated Kurdish camps high in the mountains of Iraq.

By August, the squadron had again reinforced and embarked aboard USS Wasp as the ACE for Special Purpose Marine Air Ground Task Force (SPMAGTF) Caribbean and subsequently deployed in support of Operation Support Democracy and Operation Uphold Democracy in Haiti.

HMM-264 provided the first NATO TACAIR assets to operate ashore in Bosnia-Herzegovina since the signing of the Dayton Peace Accords.

The Black Knights conducted relief missions and facilitated the movement of congressional members and other VIPs throughout the affected area.

In early December, the squadron provided support to the MV-22 Operational Test Evaluation by conducting the first shipboard interoperability training on board USS Saipan.

In support of Operation Iraqi Freedom, the squadron flew reconnaissance and presence flights in the northern area of Iraq.

During the squadron's deployment it was the ready ARG for over a month in the Persian Gulf in support of Operation Enduring Freedom, and exercise Djibouti-Ex in Djibouti, Africa.

In late August 2005, the Black Knights returned to New River, only to be tapped for a detachment to assist in the Hurricane Katrina relief efforts.

Their first deployment in December 2010 saw a milestone for the V-22 community as an aircraft piloted by the Black Knights on 10 February 2011, broke the 100,000-flight-hour mark for the platform as a whole.