[3] Kane was cast in The Fading Light by the director Ivan Kavanagh after he spotted her in a short film, July, that was posted on YouTube.

[12] In 2018, Valene Kane played journalist Amy Whittaker who investigates the recruitment of young European women by ISIS in the 2018 thriller film Profile by Timur Bekmambetov.

[13] 2019 saw Kane in Anne Sewitsky's Sonja: The White Swan which premiered at Sundance Film Festival[14] and in BBC TV Movie Counsel, in which she played "an alpha female barrister [who] complicates her professional and personal life when she takes on a young client"[15] Kane could also be heard on the Monobox Speech Share podcast reading from Marina Carr's "Portia Coughlan".

[16] Kane's radio drama work for the BBC includes The Demon Brother and Stroma Sessions for which she won Best Supporting Performer.

[3] In 2023, Kane starred as Lady Macbeth in a "subtly mercurial performance"[17] on the Royal Shakespeare Company main stage in Stratford, England[18] in a production that was "saved by its dark atmosphere and Valene Kane’s turn as Lady Macbeth"[17] She also played Morgan le Fay, King Arthur's half-sister in The Winter King, which premiered on MGM+ in the United States and on ITVX in the UK.