While walking her guard rounds in the country, Doris Lang, the orphaned daughter of a deceased werewolf hunter, is attacked and bitten by Count Magnus Lee, a 10,000-year-old vampire lord (also known as a Noble) for trespassing in his domain.

D requests that the authorities, including Greco's father, the town sheriff, and Dr. Ferringo, should hold off Doris' incarceration at the local asylum until he kills Count Lee.

Later, Greco overhears a meeting between Count Lee's messenger and Rei, during which the former gives the latter a candle with Time-Bewitching Incense, a substance powerful enough to weaken anyone with vampire blood.

Greco, who stole the candle from Rei, then appears and uses it to weaken L'Armica; also causing harm to Doris with it due to her infection.

Rei requests that the Count give him eternal life as a member of the Nobility, but is coldly rebuffed for his past failures.

As a mutant attempts to devour D's comatose body, his Left Hand revives him just in time for him to kill the monster.

D encourages L'Armica to start anew by living as a human, but she chooses to stay behind to ensure her father's legacy ends as the castle falls apart.

[10] An English-language version of the OVA was produced in 1992 by Streamline Pictures, and was shown on the fine-arts theatrical circuit in the US in August 1992.

[11] The film was also shown several times on American television during the 1990s, including on TBS, Cartoon Network, and the Sci-Fi Channel.

Vampire Hunter D is considered a flagship title for Streamline, and was marketed in the US as "the first animated horror film for adults".

[12] In 2000, Urban Vision Entertainment, the US production partner and distributor of Vampire Hunter D: Bloodlust, re-released the OVA on a "Special Edition" bilingual DVD on 17 October containing the original Japanese audio and a Dolby Digital 5.1 remix of the Streamline dub,[13][14] as well as releasing dubbed and subtitled versions of the film on VHS.

[16] On 16 April 2015, Sentai Filmworks announced their license to the film in North America for digital and home video release.