The film stars Ewa Strömberg as Linda Westinghouse, an American who works in a Turkish legal firm.

Westinghouse has a series of erotic dreams that involve a mysterious vampire woman who seduces her before feeding on her blood.

The film's score became popular in the mid-1990s when it was included on the compilation album Vampyros Lesbos: Sexadelic Dance Party, which reached the top ten on the British Alternative charts.

[3] On a remote Turkish island, the beautiful vampire Countess Nadine Carody lures unwary victims with her seductive nightclub act and sets her sights on Linda, a young American woman working in a legal firm in Istanbul.

[5] The soundtrack was composed by Manfred Hübler, Siegfried Schwab and Jesús Franco who credited himself under the alias of David Khune.

[1] Less than a month after finishing production on Vampyros Lesbos, Franco began working on his next film, She Killed in Ecstasy (1971).

"[10] Jonathan Rosenbaum of The Chicago Reader gave the film a negative review, comparing director Jesús Franco to Ed Wood.

[14] In his 2009 book The Pleasure and Pain of Cult Horror Films: An Historical Survey, Bartomiej Paszylk took umbrage with some of the high-brow critics of the film, though ultimately acquiescing to its shortcomings, "Truth be told, Franco's vampyros are far more interested in being lesbos than in drinking human blood, but the movie is so mesmerizing and so outright sexy that you really shouldn't mind that.

[17] Franco repurposed music from these albums as the soundtracks for three of his films: Vampyros Lesbos, She Killed in Ecstasy and The Devil Came from Akasava.

[17] The track "The Lions and the Cucumber" from the album was later used again on the soundtrack of Jackie Brown by American director Quentin Tarantino, as well as in season three of The L Word (episode "Lifeline").

The album was a collection of remixes from various electronic artists including Two Lone Swordsmen, Cristian Vogel and Alec Empire who released their own mixes of the film's soundtrack.