Together with the basilar membrane, it creates a compartment in the cochlea filled with endolymph, which is important for the function of the spiral organ of Corti.

It allows nutrients to travel from the perilymph to the endolymph of the membranous labyrinth.

[1][2] Histologically, the membrane is composed of two layers of flattened epithelium.

It primarily functions as a diffusion barrier, allowing nutrients to travel from the perilymph to the endolymph of the membranous labyrinth.

The vestibular membrane may be ruptured by an increase in the pressure of endolymph in the cochlear duct.