She was the daughter of courtier baron Charles De Geer (1747–1805) and Eleonora Vilhelmina von Höpken.
Her spouse had previously been engaged to Lolotte Forssberg, the alleged daughter of her father's first wife Ulla von Liewen and Adolf Frederick, King of Sweden.
In 1823, she was appointed statsfru to the newly formed household of the queen, and was in 1829 promoted to the office of överhovmästarinna or senior lady-in-waiting.
[1] Further more, while the king himself often loosened the etiquette in his own appearances, Gyldenstolpe seems to have upheld a strict conservative etiquette at the court of the queen; at one occasion during a journey, Vilhelmina Gyldenstolpe announced that while the king planned to greet also people normally not received at court because of their rank, such a thing could of course be unthinkable for the queen.
[2] Queen Désirée compared her unfavorably to the senior lady-in-waiting of her daughter-in-law Josephine of Leuchtenberg in a letter to her sister Julie Clary in March 1831: