In 1998 Virgil Lozano joined the now legendary Lions Den Academy, founded by UFC Hall of Famer Ken Shamrock.

In 1999 Lozano became part of the Lions Den professional MMA fighting team, which included notable fighters like Guy Mezger, Vernon White, Tra Telligman, Mikey Burnett, Pete Williams and Jerry Bohlander.

Lozano made his professional debut, as part of the Lions Den team, on August 10, 2001 against Yasuki Miyake from Japan.

Originally Lozano was scheduled to fight Phil Baroni but the match was changed after another fighter was pulled out of the event due to an injury.

On April 15, 2009 Lozano fought Hector Lombard in the first round of the Middleweight Tournament at the Lloyd Noble Center in Norman, Oklahoma, U.S.