Virginia Lee O'Brien (April 18, 1919 – January 16, 2001) was an American actress, singer, and radio personality known for her comedic singing roles in Metro-Goldwyn-Mayer musicals of the 1940s.

Among the films she appeared in at MGM were The Big Store (1941) with the Marx Brothers, Lady Be Good (1941), Ship Ahoy (1942) with Eleanor Powell and Red Skelton, Thousands Cheer (in which she endured ribbing from Mickey Rooney about her singing style), Du Barry Was a Lady (with Skelton and Lucille Ball), the film version of Meet the People with Dick Powell, The Harvey Girls (with Judy Garland), and Ziegfeld Follies.

She was among the stars in a 1972 nostalgia revue entitled The Big Show of 1928 with Allan Jones, Cass Daley, Beatrice Kay, and Sally Rand, which toured the country and played New York's Madison Square Garden.

In 1984, she created a cabaret act, "Virginia O'Brien Salutes the Great MGM Musicals," which was recorded at the Masquer's Club in Hollywood, and is currently available on CD and on iTunes.

She performed several times at such clubs as Hollywood's Roosevelt Hotel Cinegrill, the Vine St. Bar and Grill, and the Gardenia, as well as the Plush Room in San Francisco.