Known as Indian River Road, the state highway runs 2.32 miles (3.73 km) from SR 168 in Norfolk east to a continuation of Indian River Road at the boundary between the independent cities of Chesapeake and Virginia Beach.

SR 407 connects U.S. Route 460 (US 460) and US 13 on the south side of the Eastern Branch Elizabeth River.

Though not designated as such by the Virginia Department of Transportation (VDOT) as such, Indian River Road west of here and Berkley Avenue to the SR 337 intersection is signed as a part of SR 407.

[2] SR 407 heads east as a six-lane divided highway that crosses over Steamboat Creek and passes the former Ford assembly plant before entering the city of Chesapeake by crossing over a Norfolk Southern Railway line.

The state highway passes through residential neighborhoods and crosses the Indian River before reaching its eastern terminus at MacDonald Road, which follows the boundary between Chesapeake and the city of Virginia Beach.