[3] It is found in Amazonian Brazil, Colombia, Ecuador, and Peru, and in southern Guyana, eastern Suriname, and French Guiana.

Vitreorana ritae is a tiny glass frog that lacks humeral spines in males and has a lobed liver.

The translucent eardrum is visible but not large, measuring about one-fourth to one-third of the eye's diameter; the tympanic annulus is not hidden except for the dorsal margin which is covered by the supratympanic fold.

This species has many clear and fine blackish spots decorating its upperside, which may appear as if they were suspended a bit above the greenish background in very weakly pigmented specimens.

The forward quarter to half of the parietal (outer) peritoneum is white, while the rest is transparent, allowing to see the frog's interior.

The iris is greyish white with a network of thin dark grey lines; in Helena's glass frog it is bright yellow.

With a considerable range including several protected areas and apparently able to live in secondary forest and tolerate some amount of human use of its habitat, it is not considered threatened by the IUCN.