The Vocation of the Apostles is a fresco by the Italian Renaissance painter Domenico Ghirlandaio, executed in 1481–1482 and located in the Sistine Chapel, Rome.

Two frescoes are certainly by Ghirlandaio, the Vocation of the Apostles and the Resurrection, which was repainted in the late 16th century due to extensive damage.

A third, The Crossing of the Red Sea, in the "Stories of Moses" wall, has been assigned to one among Ghirlandaio, Biagio d'Antonio or Cosimo Rosselli.

At the left is a white bearded man, perhaps a literate from Constantinople who was also used as a model for St. Jerome in His Study in the church of Ognissanti in Florence.

At the center, just behind Jesus, is the portrait of Diotisalvi Neroni, who had taken refuge in Rome after plotting against Piero di Cosimo de' Medici.