The show stars Steve-O, Chris Pontius, Jason "Wee Man" Acuña, Preston Lacy, Ehren McGhehey and Dave England, who perform stunts and acts with animals, often putting themselves in situations for which they are not trained.

Wildboyz follows the antics of mainly Chris Pontius and Steve-O along with Wee Man, Preston Lacy, Ehren McGhehey and Dave England as they travel around the globe.

In the course of the show, Steve-O purposely subjected himself to the relatively mild sting of the emperor scorpion on more than one occasion, and Pontius was nearly attacked by a jaguar and was bitten by a black bear.

One of their most dangerous stunts showed the boys attracting a great white shark while disguised as seals, then jumping into the open water just 10m away.

Manny Puig, a wild animal expert who appeared on Jackass several times, occasionally joined the Wildboyz on their adventures.

Fellow Jackass cast members Johnny Knoxville, Wee Man and Loomis Fall also made guest appearances on the show throughout all four seasons.