He played college football for the Penn State Nittany Lions and Kentucky Wildcats before being selected by the Titans in the second round of the 2023 NFL draft.

Levis transferred to the University of Kentucky in 2021,[7] after having graduated that May with a bachelor's degree in finance from Penn State's Smeal College of Business.

In Week 8, on October 29, 2023, with Tannehill sidelined by an ankle injury, Levis made his NFL debut and first start, finishing with 238 passing yards and four touchdowns in a 28–23 win over the Atlanta Falcons.

[18] In Week 10, on November 7, 2023, head coach Mike Vrabel announced that Levis would be the starting quarterback for the Titans moving forward, benching Tannehill.

[21] Levis became the Titans' starting quarterback for the 2024 season following the departures of Tannehill and Willis, with newly signed Mason Rudolph named as backup.

[30] Levis' improvement continued the following week, leading the Titans to a 32–27 upset against the rival Houston Texans, completing 18 out of 24 passes for 278 total yards, 2 touchdowns, and 1 interception.

[31] On Week 13, Levis completed 18 of 37 passes for 212 yards, two touchdowns, and no turnovers during the following road match against the Washington Commanders, a mixed performance on a harsh 19–42 loss that was marked by team fumbles and a 0–28 deficit in the first half.

[33][34] Levis was subsequently benched the next two games, but he alternated with Rudolph in the season closer against the Houston Texans, completing 9 out of 17 passes for 175 yards and a touchdown.

[37] Levis's father, Mike, played football at Denison University, an NCAA Division III school in Ohio, while his mom, Beth Kelley, was a two-time All-American soccer player at Yale.