William Rudolf (June 6, 1791 – January 1, 1859) was a merchant and political figure in Nova Scotia.
He operated a store in Lunenburg and owned a number of ships which traded in goods from the West Indies.
Rudolf served his community as justice of the peace, postmaster, registrar of deeds and lieutenant-colonel in the local militia.
In 1838, he was named to the Legislative Council, serving as a member until his death in Lunenburg at the age of 67.
His widow, Anna Matilda Rudolf, served several years as postmaster for Lunenburg and died in 1886.