It is shown to be sufficient and necessary for apical RNA transport in microinjection assays and transgenic reporters.

[1] Indeed, deletion of WLE3 in an otherwise full-length wg 3'UTR completely abolishes apical localization.

However, a minimal WLE3 monomer by itself shows weak activity on its own.

Phylogenetic comparison of WLE3 elements predicts a highly conserved stem-loop structure in Drosophila species (using ALIFOLD) and deemed necessary for WLE function by mutational analyses.

Targeted mutations on the sequence and structural aspects of WLE3 stem-loop were done and tested showing the distal stem to be most important, the central variable region while must be present its sequences and bulges appear to be unimportant and the WLE3 loop with two invariant residues is not required for localization.