The 65th Assembly district of Wisconsin is one of 99 districts in the Wisconsin State Assembly.

[1] Located in southeast Wisconsin, the district comprises part of southeast Kenosha County, including the southern half of the city of Kenosha and the northern half of the village of Pleasant Prairie.

The district also contains downtown Kenosha, Kenosha Harbor, and landmarks such as Civic Center Historic District and Library Park.

[2] The district is represented by Democrat Ben DeSmidt, since January 2025.

[3] The 65th Assembly district is located within Wisconsin's 22nd Senate district, along with the 64th and 66th Assembly districts.