It runs in north–south in east central Wisconsin from the unincorporated community of Waukau to Butte des Morts.

Heading north through the town, it continues in a generally northern direction until it reaches CTH-E (Fourth Street Road) on the outskirts of Omro.

Once in Omro, WIS 116 heads north on Jefferson Avenue to Main Street, on the south side of the Fox River.

Continuing northwest out of the city, WIS 116 becomes Achterberg Road until it reaches Main Street in Winneconne.

Crossing over the Wolf River in downtown Winneconne, WIS 116 heads east to its terminus at the interchange with US 45 near the northern shore of Lake Butte des Morts.