The route serves local traffic in the western part of the state, connecting Durand, Menomonie and Barron.

WIS 25 begins at the Minnesota state line in Buffalo County on the Mississippi River.

[4] WIS 25 passes west of Dallas, and through the small community of Hillsdale before entering Barron thirteen miles (19 km) north of the county line.

[1][7] The route was extended again to Nelson when WIS 35 was rerouted across a new bridge over the Chippewa River in 1933.

Finally in 1947, The route was extended through Nelson and across the Mississippi River and connected to MN 60.