WI 64 is a limited-access freeway from 150th Avenue in Houlton until 85th Street and Raleigh Road in Somerset.

Continuing east, the highway is an expressway with at-grade intersections until 145th Street in New Richmond.

In Cylon, Wisconsin WIS 64 runs concurrently with US 63 from the US 63/WIS 46 (known locally as 'Four Corners') junction until 260th Street.

WSI 64 winds through Dunn County, running through mostly rural areas and unincorporated towns.

Approximately half-way through its routing in the County, WIS 64 runs concurrently with Wisconsin Highway 25.

Highway 64 continues east through Chippewa County, crossing US 53 at exit 112, just north of Bloomer.

The two state highways run concurrently out of the city to the Cornell Municipal Airport.

WIS 64 runs along the southern border of Chequamegon National Forest en route to Medford, where it becomes Broadway Avenue.

WIS 64 continues slightly northwest into Goodrich before entering Lincoln County.

WIS 64 continues due east, going through the towns of Polar, Elton, White Lake, and Langlade.

US 41 continues east on Hall Avenue to Bridge Street and into the state of Michigan.