The team plays their home games at Neuroscience Group Field at Fox Cities Stadium, which opened in 1995.

Appleton, the largest of Wisconsin's Fox Cities, has hosted Minor League Baseball teams since the late 19th century.

The city's professional baseball history dates back to 1891 with the formation of the Appleton Papermakers in the single-season Wisconsin State League.

[3] Managed by future Baseball Hall of Famer Earl Weaver,[8] they won the Three–I League championship pennant with a league-best 82–56 record in their first season with the Orioles.

[4] Manager Billy DeMars led the 1964 Foxes to win the second half title, qualifying them for a single championship game against the Clinton C-Sox.

Stan Wasiak managed the 1966 squad to the first half title and a 2–0 championship series win over the Cedar Rapids Cardinals.

[16][17][18] In 1967, then known as the Appleton Foxes,[4] Manager of the Year Alex Cosmidis' team won the second half title before sweeping the Wisconsin Rapids Twins in two games to win the MWL crown again.

[13][19][20] Two years later, Tom Saffell's Foxes won both halves of the 1969 season and were named league champions without any playoffs being held.

[4] Having won the first half, they went on to capture the Northern Division title versus the Waterloo Indians, 2–0, before beating the Burlington Bees, 2–1, to win their fifth MWL championship.

Clinching a wild card berth in 1982, Appleton won the semifinals against the Springfield Cardinals, 2–0, then claimed the league title over the Madison Muskies, 2–1,[27] under manager Adrian Garrett.

[31] Sal Rende's 1984 Foxes completed the back-to-back-to-back feat by winning the division, defeating Madison, 2–1, in the semifinals, and again taking the championship from Springfield in a full five-game series.

[37] The name refers to the timber rattlesnake, which is not typically found in the Appleton area but is more common in southwest Wisconsin.

[38][39] The team's scheduled April 5, 1995, home opener at their new facility was postponed due to snow and rescheduled for the next afternoon as a doubleheader.

[41] In 1994, shortstop Alex Rodriguez was selected as the league's Prospect of the Year after hitting for a .319 batting average with 14 home runs and 55 RBI in just 65 games.

As first-half winners in 1996, they won the Central Division title versus the Peoria Chiefs, 2–1, and then beat the Quad Cities River Bandits, 2–1, to advance to the championship round, but they were defeated by West Michigan, 3–1.

In 1999, the Timber Rattlers qualified for the postseason with a second half title, won the Central Division over the Rockford Reds, 2–0, advanced through the semifinals over the Lansing Lugnuts, 2–0, but again lost the championship to Burlington, 3–2.

In 2005, Wisconsin won both halves, the quarterfinals over the Beloit Snappers, 2–1, and the Western Division title against the Clinton LumberKings, 2–0, but failed to win the league championship as they were defeated by South Bend, 3–2.

[48] That same season, the franchise won the Larry MacPhail Award for outstanding minor league promotions.

[53] In 2022, the High-A Central became known as the Midwest League, the name historically used by the regional circuit prior to the 2021 reorganization.

[57] They won the Western Division title over the Quad Cities River Bandits, 2–0,[58] but were defeated in the championship series by the Lake County Captains, 2–1.

[61] Over 15 complete seasons of competition as a Brewers farm club, the Timber Rattlers hold a regular-season win–loss record of 976–1,057.

[142] Fang is joined by Whiffer, a secondary mascot whose appearance resembles that of the Phillie Phanatic with teal fur and green face and hands.

[144] Circa 1980, the mascot was Homer Run, who was human in appearance and wore the same style uniform as the Foxes.