The Port Stanley Lakers were founded in 1989 as members of the Western Ontario Junior D Hockey League.

In 1994, the Lakers finished first amongst all OHAJDL teams in the regular season with a stellar record of 36 wins, 2 losses, and 2 ties.

They made it all the way to the league finals in the playoffs and found themselves nose-to-nose with the Mitchell Hawks.

The Hawks were not to be denied as they swept the Lakers aside 4-games-to-none to win the OHA Cup as league champions.

After finishing first overall in the OHAJDL with a record of 31 wins and 9 losses, the Lakers pushed deep into the playoffs.

The Lakers challenged the Burford Bulldogs and defeated them 4-games-to-2 to win their franchises first ever OHA Cup.

After finishing first overall in the league, the Lakers entered the playoffs against the North Middlesex Stars.

In the off-season, the OHAJDL disbanded and the Southern Ontario Junior Hockey League was formed in its place.

After nineteen seasons in the Ontario Hockey Association, the Lakers franchise announced that they will be leaving the OHA.

"The Lakers will continue play in various forms at other venues outside the OHA," said team President Wayne Bedford.

The Lakers gained international attention due to a video recorded during a road game against the Kingsville Kings on November 20, 2015.

A brawl broke out when a London player struck Kingsville's goalie Jan Pechek.

Following the 2022–2023 season, the Lakers re-located to Woodstock, Ontario and play their home games at Southwood Arena.