[10][11] Yesung officially debuted as part of 12-member project group Super Junior 05 on November 6, 2005, on SBS's music programme Popular Songs, performing their first single, "Twins (Knock Out)".

The trio debuted on November 5, 2006, on KBS's Music Bank with performing Hyena soundtrack called "The One I Love".

[19] He debuted as an actor on July 26, 2007, with the release of Attack on the Pin-Up Boys, a high school comedy starring fellow Super Junior members.

[20] On May 9, 2008, Yesung was sent to the hospital after he injured his leg during the 70 km 24-Hour Marathon of Hope in which he and bandmates Shindong, Sungmin, Leeteuk, Eunhyuk and Kangin participated.

The event was in collaboration with the SBS charity program "Hope TV 24" and the money raised was donated to a Mongolian school in South Korea which was at risk of closing down.

[21] Due to the injury, Yesung was unable to complete the marathon, but appeared on stage on crutches with his fellow members.

He sang ballad track "Love Really Hurts" for the original soundtrack of television drama Tazza, which aired from September 16, to November 25, 2008.

Yesung played villain "Jung Myung-soo", a servant-turned-interpreter who feels sad from being betrayed by his country, from October 9 to November 4, at the Seongnam Arts Center Opera House.

[25] On November 6, he made a surprise appearance on KBS' Music Bank with label mates SHINee in place of Jonghyun, who was recovering from influenza, in a performance of "Ring Ding Dong".

[27] On March 31, 2010, Yesung contributed to the OST of television drama Cinderella's Sister, starring Moon Geun-young and Chun Jung-myung.

On September 4, he became the new MC for MUZIT, a musical talk show alongside K.Will and veteran composer Yoo Young-suk.

[32] On December 29, 2010, Yesung together with Luna of f(x), sang the track "Loving You", on part two of the original soundtrack of KBS drama President.

The song has a fantastic harmony and is mixed with the lyrics of secretly loving someone and wondering if that person could possibly be feeling the same way about them.

During the recording of Dream Team 2 on August 21, 2011, he fell from a platform and strained his waist again, but was reported to be a minor injury.

[42] On October 23, 2011, Yesung as part of Super Junior performed in the New York SM Town concert at Madison Square Garden.

[43] On November 11, 2012, Yesung along with his brother Kim Jong-jin officially opened their café "Mouse Rabbit Coffee" after their franchise, "Babtols" and "Handel & Gretel".

On February 13, 2013, Yesung's OST "Gray Paper" for SBS melodrama That Winter, the Wind Blows was released.

The track was composed by label mate Kangta and where he went on to performe it on SBS's music program Inkigayo on February 17.

On the group's second album, he had a solo song for the track "Blind", which releasing on Korean and Japanese version sung by Yesung, however didn't participate with the promotions.

A Japan Tour was started in Yokohama on June 2 to 3 and had 11 total performances in arenas in Kobe, Fukuoka, and Nagoya.

[57] In August, Yesung solo song Dreaming from the OST of MBC drama, Splendid Politics was released.

[66] He also began his tour, Super Junior-Yesung Special Live – Y's STORY, in February 2019, with stops at Tokyo, Osaka, Nagoya and Fukuoka.

[68] On May 3, 2021, Yesung released his fourth extended play Beautiful Night, consisting of seven tracks, including the lead single of the same name.

[71] A week later, the album's name is revealed to be Sensory Flows and scheduled to be released on January 25 with its lead single "Small Things".

On May 24, he released his first Japanese EP, Kimi to Iu Sakura no Hanabira ga Boku no Kokoro ni Maiorita.