Yossef Harmelin (Hebrew: יוסף הרמלין; 1922 – December 12, 1994) was an Israeli civil servant, serving as the director of the Shabak from 1964 to 1974 and again from 1986 to 1988 and as ambassador in Iran and South Africa.

He was first at Ben Shemen Youth Village and then founded kibbutz Neve Yam.

Harmelin fought with the Israel Defense Forces in the 1948 Arab–Israeli War.

In 1974, he left Shabak to pursue other interests, including his ambassadorship.

He was the last Israeli ambassador to Iran, before diplomatic relations were severed following the Iranian Revolution.