[1] In 1999, an instance of police repression against sex workers prompted Rotela to dedicate herself to activism, publicly denouncing the abuses and extortion that affect this community.

As a sex worker, she began to organize with her colleagues, and in 2007, she co-founded Panambí: Association of Transvestite, Transsexual, and Transgender People, which adopted its charter in 2008 and obtained legal status in 2009.

Rotela participated in the 47th General Assembly of the Organization of American States, held in Cancún, Mexico, as the Paraguayan representative of the Trans Persons Network of Latin America and the Caribbean (Redlactrans).

[8] Part of her platform was based on "recognizing the gender identity of trans people, access to economic, social, cultural, civil, and political rights for the entire LGBT community, and the approval of a law against all forms of discrimination.

[10] In December 2016, through a civil trial, Yren Rotela became the first trans person, together with Mariana Sepúlveda, to present a request to change her name in Paraguay.