Yuki Mizoguchi (aka Mizoguchi Yuki) was born in Ōtsu city, Shiga Prefecture and graduated from the Nara Women's Normal School (the predecessor of Nara Women's University).

She was employed as a school teacher, but her interest in art led her to study under noted Nihonga painter Yukihiko Yasuda in 1920.

In 1926, her painting Kyuri ("Cucumbers") was selected for an Inten Exhibition by the Japan Fine Arts Academy (Nihon Bijutsu-in).

She specialized in graceful family scenes, still life, and pictures of women.

A prolific artist, in 1976, she was selected to become a member of the Japan Art Academy (Nihon Geijitsu-in), and later become honorary chairperson of that organization.