At that time he was inspired by the science fiction novel Stellar Ships by the Soviet writer and paleontologist Ivan Yefremov (where it was written about a three-dimensional image of alien head emerged in the ancient disc made of unknown material), and remarkable experiments by 1908 Nobel Prize laureate Gabriel Lippmann.

In 1958, in other words before the times when lasers with their coherent light were invented, he started conducting his own experiments, where he used lamp emission on mercury vapor and was first to demonstrate 3D hologram.

In 1962 Denisyuk invented the method of image recording in three-dimensional environments allowing one to save information about phase, amplitude, and the spectral structure of the wave coming from an object.

This was determined by the government to be a scientific discovery, and was registered with the USSR State Committee on Inventions and Discoveries under No.88 as of February 1, 1962 along with the following statement: “The established is an unknown before phenomenon of spatial distortion-free colored image object occurrence under illumination from the three-dimensional element of the transparent material medium, in which the density distribution of matter matches the intensity extension of standing-wave field, which are formed around the object at radiated emission scattering on it”[This quote needs a citation] Denisyuk received the Lenin Prize in 1970, upon receipt of the prize he was selected as a member of the Academy of Sciences of the Soviet Union, and appointed as a head of the newly established Laboratory of holography at Vavilov State Optical Institute.

A great deal of his scientific work was dedicated to examination of quasi-deep holograms (the specific one-dimensional structure) and selectograms (a new type of periodic three-dimensional environment).