Zinc finger protein 800

The ZNF800 gene is between 127373344bp and 127391557bp on the reverse strand of Chromosome 7, locus 7q31.33, spanning a total of 18214 base pairs.

ZNF800 contains 6 functioning C2H2 zinc finger protein domains, a number of SNP's, and multiple experimentally-shown phosphorylation[2][3][4] and SUMOylation sites.

[5][6][7][8][9] ZNF800 is in the neighborhood of PAX4,[10] which plays an important role in the differentiation and development of pancreatic islet beta cells.

ZNF800 is ubiquitously expressed in at least 27 different tissues, with the largest amounts in bone marrow, testis, and lymph nodes.

Several different tools were used on ExPASy Proteomics to analyze ZNF800 for likely protein modification sites, unique composition, and localization.

The likely sites of Phosphorilation, O-Glycosilation, Sumoylation, and O-ß-GlcNAc attachment are detailed below: (N/A when applied to whole protein) pat4: RKPK (4) at 473 pat4: RRKR (5) at 529 pat7: none bipartite: RRGVRRHIRKVHKKKME at 242 bipartite: KRDVIRHITVVHKKSSR at 531 content of basic residues: 17.8% NLS Score: 1.27 RRGVRRHIRKVH at 232

When multiple sequence alignments were made, the zinc finger binding domains were the areas with the most conservation.

Based on the found E values of the protein with its orthologs in the aforementioned categories using NCBI Blast,[23] ZNF800 is at least 930 Millions of years old.

At first it was hypothesized that ZNF800 has an Ortholog in fungus, dating back to 1150 Millions of years ago, however, a BLAT[10] search of the fungus sequence in the human domain gave no results, which lead to the conclusion that these sequences are not similar enough to prove they are truly related.

The most common transcription factors with high probability (>0.84) of binding ZNF800 promoter are shown in the figure.

There are 4 existing patents mention ZNF800 in lists of 100s-1000s, which address concepts such as “prostate cancer progression”, “progression risk of glaucoma”, “method for inducing pluripotency in human somatic cells”, and “modifying transcriptional regulatory networks in stem cells”.

ZNF800 is found at locus 7q31.33.
This conceptual translation highlights the zinc finger domains, phosphorylation sites, sumoylation sites, SNP's, and important DNA sequences of human ZNF800.
Potential layout of the zinc finger protein 800 with significant regions highlighted, such as conserved areas of unknown function in grey, zinc finger regions in green, and unstructured region upstream of the zinc finger in blue.
Graph of tissue specific expression of ZNF800 in humans from The Human Protein Atlas. [ 13 ]
This graph shows levels of ZNF800 in human tissues from experimental values on GEO Profile [ 14 ] accessed with "GDS 424".
This graph shows experimental tissue specific expression of ZNF800 in humans found on GEO Profiles [ 14 ] when searching "GDS 424".
Allen Brain Atlas [ 15 ] image of ZNF800 Expression in the mouse brain. This shows ubiquitous expression in low levels.
This graph was made using calculations of m and n where n is 100-% identity of a protein and m=-ln(1-n/100)*100. Identity percentages were found using BLAST. [ 22 ]
This tree from Clustal W [ 24 ] shows an unrooted phylogenetic tree of the ZNF800 orthologs showing relatedness and animal type.
[ 25 ] STRING Prediction of ZNF800 interacting proteins.
This image from GEO Profiles of ZNF800 [ 27 ] shows a correlation between expression of ZNF800 and chronic B-lymphocytic leukemia.
This image from GEO Profiles of ZNF800 [ 27 ] shows a relationship between patients with myotonic dystrophy type 2 and control. Based on the graph, control patients have significantly higher ZNF800 expression than patients with myotonic dystrophy, suggesting there may be some relationship.