Thus, on 5 December 1921, three firing batteries, A, B, and C, formed the basis of the First Battalion, 116th Field Artillery, Major Sumter L. Lowry Jr. commanding.

The Second Battalion participated in the summer encampment in 1923, and was federally recognized as a unit on 23 August 1923, with Major Fred Hampton commanding.

Ultimately, the subordinate units of the 2nd Battalion, 116th Field Artillery were re-designated and relocated in some cases in different cities: HQ Battery – Lakeland.

The regiment, less two batteries, was used for riot control duties in connection with the protection of prisoners at the Hillsborough County Jail in Tampa, from 31 May to 6 June 1927.

Several batteries were used for guard duties in connection with the visit of President Franklin D. Roosevelt to Winter Park, Florida, on 31 March 1936.

[2] The 2nd Battalion, 116th Field Artillery has seen service in Iraq and Afghanistan and served in Homestead, Florida in the aftermath of Hurricane Andrew.