The 140th Indiana Infantry Regiment served in the Union Army between October 24, 1864, and July 11, 1865, during the American Civil War.

From Tennessee they moved to Washington, D.C.; then to Fort Fisher, North Carolina, remaining there until February 7.

From February 18 to 20, the regiment moved to Fort Anderson and from there to Town Creek.

On April 26, at Bennett Place, the regiment accepted the surrender of General Joseph E. Johnston and his army.

Following the surrender, the regiment saw duty at Raleigh until May 6, and at Greensboro until early July.