The 16th Regiment Illinois Volunteer Cavalry was a cavalry regiment that served in the Union Army during the American Civil War.

The 16th Illinois Cavalry was organized at Camp Butler, Illinois, between January and April 1863 from numerous independent companies of Illinois cavalry.

The regiment mustered out on August 19, 1865 .

The regiment suffered 3 officers and 30 enlisted men who were killed in action or who died of their wounds and 1 officer and 228 enlisted men who died of disease, for a total of 262 fatalities.

This article about a specific military unit of the American Civil War is a stub.