Defending champion René Lacoste defeated Bill Tilden in the final, 11–9, 6–3, 11–9 to win the men's singles tennis title at the 1927 U.S. National Championships.

[2] It was Lacoste's second U.S. Championships singles title and fifth major singles title overall.

[3] The tournament used two lists of eight players for seeding the men's singles event; one list of U.S. players and one for foreign players.

René Lacoste is the champion; others show in brackets the round in which they were eliminated.