The Serie B 1934–35 was the sixth tournament of this competition played in Italy since its creation.
Eight teams for each group were relegated in order to restore the single table.
Two teams (one for each group) retired during the tournament.
In the Western group, A.C. Pavia retired after 20 days and only the matches played in the first half of the season (the andata) were considered valid.
Played in Ancona on June 9 Played in Fano on June 16 U.S. Cremonese were relegated to Serie C. Genova 1893 were declared Serie B champions.