1959–60 Tweede Divisie

Eight teams would play against relegation this season in a play-off.

Two teams already had to play in it on the basis of last season's results: ONA and Velocitas 1897.

The other six participants were the lowest ranked teams of this season.

The changes were part of an attempt by the KNVB to make the Tweede Divisie one league next year.

Also, one extra team would be promoted to the Eerste Divisie this year, through a play-off between the third-place finishers of both groups.