The 1990 Eastern Kentucky Colonels football team represented Eastern Kentucky University as a member of the Ohio Valley Conference (OVC) during the 1990 NCAA Division I-AA football season.
Led by 27th-year head coach Roy Kidd, the Colonels compiled an overall record of 10–2, with a mark of 5–1 in conference play, and finished as OVC co-champion.
Eastern Kentucky advanced to the NCAA Division I-AA First Round and were defeated by Furman.
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