The 1999–2000 Butler Bulldogs men's basketball team represented Butler University in the 1999–2000 NCAA Division I men's basketball season.

Their head coach was Barry Collier, serving in his 11th and final season at the school.

The Bulldogs played their home games at Hinkle Fieldhouse as members of the Midwestern Collegiate Conference.

12 seed in the East region, and entering play with a 15-game win streak, the Bulldogs were beaten at the buzzer by No.

5 seed Florida, 69–68 in OT,[1] in a game the Gators used as a springboard to an eventual National runner-up finish.