The 2000–01 Butler Bulldogs men's basketball team represented Butler University in the 2000–01 NCAA Division I men's basketball season.

The Bulldogs played their home games at Hinkle Fieldhouse as members of the Midwestern Collegiate Conference.

Butler finished first in the MCC season standings and won the MCC tournament to receive the conference’s automatic bid to the NCAA tournament – the school’s fourth NCAA Tournament appearance in five years.

7 seed Wake Forest, 79–63,[1] in the opening round, before falling to No.

For the second straight season, Butler lost to the eventual National runner-up.