The 1st Florida Cavalry Regiment was a cavalry regiment from Florida that served in the Union Army from October 29, 1863 – November 17, 1865 during the American Civil War.

Recruitment and organization of the unit began at Fort Barrancas and lasted from December, 1863 to August, 1864.

[citation needed] The unit was composed primarily of men from southern Alabama and northwestern Florida and 81% of the initial recruits came from only nine counties in the two aforementioned regions.

In 1865, the unit went on a February 22–25 expedition to Milton, Florida before taking part in the March 18 – April 9 campaign against Mobile, Alabama and its defenses.

After the occupation of Mobile on April 12, the regiment marched towards Montgomery, Alabama.