As part of the Army of Northern Virginia it fought in the Eastern Theater until the surrender at Appomattox.

Colonel Montford S. Stokes, a son of North Carolina Governor MontfortStokes, became its commanding officer.

The regiment initially served in Department of North Carolina, and after the First Manassas was relocated to Virginia.

[1] As part of the Second Corps, the 1st North Carolina participated in almost every battle the Army of Northern Virginia fought in the Eastern Theater.

[4] The regiment surrendered with the army at Appomattox on April 9, 1865; fielding 10 officers and 61 enlisted men.