The 2000–01 Purdue Boilermakers women's basketball team represented Purdue University as a member of the Big Ten Conference during the 2000–01 NCAA Division I women's basketball season.

Led by second-year head coach Kristy Curry, the Boilermakers played their home games at Mackey Arena in West Lafayette, Indiana.

The Boilermakers beat UC Santa Barbara, LSU, Texas Tech, and Xavier to reach their second Final Four in three seasons.

They defeated SW Missouri State in the National semifinals.

In the National championship game, Purdue lost to Notre Dame by just two points, 68–66.