The 2000 AFL draft was the annual draft of talented Australian rules football players by teams that participate in the Australian Football League.
It consisted of a pre-season draft, a national draft, a trade period and a rookie elevation.
The St Kilda Football Club received the first pick in the national draft after finishing on the bottom of the ladder during the 2000 AFL season.
In alphabetical order of new clubs In alphabetical order of professional clubs.
This list details 2000-listed rookies who were elevated to the senior list; it does not list players taken as rookies in the rookie draft which occurred during the 2000/01 off-season.