The 2000 Conference USA baseball tournament was the 2000 postseason baseball championship of the NCAA Division I Conference USA, held at Florida Power Park in St. Petersburg, Florida, from May 17 through 21.
Houston defeated Cincinnati in the championship game, earning the conference's automatic bid to the 2000 NCAA Division I baseball tournament.
The play-in round that had been used to determine which of the lowest seeds would participate in the eight-team bracket was eliminated.
Instead, only the top eight of the conference's ten teams in the regular season qualified for the tournament.
The loss of the play-in round shortened the tournament from six to five days.