The 2000 NAPA Auto Parts 500 was the tenth stock car race of the 2000 NASCAR Winston Cup Series and the fourth iteration of the event.

Team Menard's Robby Gordon set the fastest time in the session, with a lap of 38.866 and an average speed of 185.252 mph (298.134 km/h).

[3] The final practice session, sometimes referred to as Happy Hour, was held on Saturday, April 29, after the preliminary 2000 Auto Club 300.

Penske-Kranefuss Racing's Jeremy Mayfield set the fastest time in the session, with a lap of 39.960 and an average speed of 180.180 mph (289.972 km/h).

[4] Mike Skinner, driving for Richard Childress Racing, managed to win the pole, setting a time of 38.697 and an average speed of 186.061 miles per hour (299.436 km/h) in the first round.

The race was aired live on ABC in the United States for the twenty ninth and final time Bob Jenkins, 1973 Cup Series champion Benny Parsons and two-time NAPA Auto Parts 500 winning crew chief Ray Evernham called the race from the broadcast booth.