The 2000 NCAA Division III men's basketball tournament was the 26th annual single-elimination tournament to determine the national champions of National Collegiate Athletic Association (NCAA) men's Division III collegiate basketball in the United States.

The field contained forty-eight teams, and each program was allocated to one of four sectionals.

All sectional games were played on campus sites, while the national semifinals, third-place final, and championship finals were contested at the Salem Civic Center in Salem, Virginia.

[1] Calvin defeated Wisconsin–Eau Claire, 79–74, in the championship, clinching a second national title.

Sherm Carstensen of Wisconsin-Eau Claire was named Most Outstanding Player after scoring 34 points in the semifinal and 36 in the championship game.