The 2005 Nicholls State Colonels football team represented Nicholls State University as a member of the Southland Conference during the 2005 NCAA Division I-AA football season.

Led by second-year head Jay Thomas, the Colonels compiled an overall record of 6–4 with a mark of 5–1 in conference play, sharing the Southland title with Texas State.

Nicholls State advanced to the NCAA Division I-AA Football Championship playoffs, losing to Furman in the first round.

The team played home games at John L. Guidry Stadium in Thibodaux, Louisiana.

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