The current champions are Metz who won the competition by defeating Sochaux 4–3 on penalties in the final after the match ended 1–1 in 90 minutes.
The draw for the Round of 32 of the Coupe Gambardella was conducted on 4 February 2010 at the headquarters of Crédit Agricole, who sponsor the competition, in Paris by the France national football team manager Raymond Domenech and a host of members of the Ligue du Football Amateur, including the president Fernand Duchaussoy.
The draw for the Round of 16 of the Coupe Gambardella was conducted on 25 February 2010 at the Ligue de Paris-Île-de-France headquarters, in Paris, by France national under-18 football team manager Philippe Bergeroo and Jean-Claude Giuntini, a member of the Ligue du Football Amateur.
The draw for the Round of 16 of the Coupe Gambardella was conducted on 25 February 2010 at the headquarters of Crédit Agricole, who sponsor the competition, in Paris by French parachuting champion Nicolas Ratier.
The draw for the Round of 16 of the Coupe Gambardella was conducted on 25 February 2010 at the headquarters of Crédit Agricole, who sponsor the competition, in Paris by French parachuting champion Nicolas Ratier.