The 2009–10 Hawaii Rainbow Wahine basketball team represented the University of Hawaiʻi at Mãnoa in the 2009–10 NCAA Division I women's basketball season.

The Rainbow Wahine, coached by Dana Takahara-Dias, are a member of the Western Athletic Conference.

The Jack in the Box Rainbow Wahine Classic will be held from November 27–29.

On December 19, the school will participate in the Duel in the Desert.

From December 29–31, the Rainbow Wahine will play in the Waikiki Beach Marriott Resort Classic.